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Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling

Bhaskar Chanda Stem Cell Múnera et al. report the generation of pluripotent stem cell (PSC)-derived human colonic organoids (HCOs). They first find that a conserved BMP-HOX pathway is required to establish posterior identity and then show that activating BMP signaling in human PSC-derived CDX2+ gut tube spheroids generates HCOs that retain colonic identity after transplantation.

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Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State

Bhaskar Chanda Stem Cell In this article, Deng and colleagues show that functional neurons and hepatocytes can be induced from fibroblasts via a chemically induced and highly expandable XEN-like state, bypassing the pluripotent stage. This featured lineage reprogramming strategy would be generalized for generating other functional desirable cell types.

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Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State

Bhaskar Chanda Stem Cell In this article, Deng and colleagues show that functional neurons and hepatocytes can be induced from fibroblasts via a chemically induced and highly expandable XEN-like state, bypassing the pluripotent stage. This featured lineage reprogramming strategy would be generalized for generating other functional desirable cell types.

from Cell Stem Cell http://ift.tt/2sYuUQr
via Bhaskar Chanda UHN

Dynamic Reorganization of Chromatin Accessibility Signatures during Dedifferentiation of Secretory Precursors into Lgr5+ Intestinal Stem Cells

Bhaskar Chanda Stem Cell Jadhav et al. identify an active enhancer signature that distinguishes Lgr5+ intestinal stem cells (ISCs) from Bmi1GFP+ and other secretory cells, including CD69+CD274+ goblet cell precursors. These specialized cells dedifferentiate into Lgr5+ ISCs in response to stem cell attrition, which is accompanied by dynamic rearrangements in open chromatin signatures.

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Integrin α7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma

Bhaskar Chanda Stem Cell Haas et al. identify integrin α7 as a functional marker of glioblastoma stem cells by screening a monoclonal antibody library generated against primary glioblastoma (GBM) cells. Functional experiments in culture and in vivo show that the targeting of integrin α7 has potential as a therapeutic avenue for treating this aggressive disease.

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Integrin α7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma

Bhaskar Chanda Stem Cell Haas et al. identify integrin α7 as a functional marker of glioblastoma stem cells by screening a monoclonal antibody library generated against primary glioblastoma (GBM) cells. Functional experiments in culture and in vivo show that the targeting of integrin α7 has potential as a therapeutic avenue for treating this aggressive disease.

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Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier

Bhaskar Chanda Stem Cell Vatine et al. show that human iPSC-based modeling can pinpoint the origin of a neuronal disorder in the brain as a defect in transport of thyroid hormone across the blood-brain barrier, rather than in the neurons themselves.

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Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution

Bhaskar Chanda Stem Cell The olfactory epithelium is a site of active neurogenesis. Fletcher et al. combine single-cell transcriptomics and clonal lineage analysis to trace cell fates from the multipotent olfactory stem cell and identify multiple mechanisms controlling cell fate, including direct conversion of quiescent stem cells into support cells without cell division.

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Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy

Bhaskar Chanda Stem Cell Lechner et al. identify regenerative myeloid subpopulations, including recruited CCR2+ monocytes and Arginase1+ macrophages, during pneumonectomy-induced lung regeneration. Loss of recruited monocytes or Il4ra signaling impaired lung regeneration. The authors provide evidence that ILC2s and lung macrophages modulate the regenerative microenvironment to support alveolar epithelial stem cell proliferation and differentiation.

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An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice

Bhaskar Chanda Stem Cell Klein and colleagues show, using the mouse incisor as a model, that the transcriptional cofactors YAP and TAZ, components of the Hippo pathway, regulate stem cell-based tissue renewal by controlling the proliferation and differentiation of transit-amplifying cells in response to integrin/FAK signaling.

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