Archive | June 2017

Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling

Bhaskar Chanda Stem Cell Múnera et al. report the generation of pluripotent stem cell (PSC)-derived human colonic organoids (HCOs). They first find that a conserved BMP-HOX pathway is required to establish posterior identity and then show that activating BMP signaling in human PSC-derived CDX2+ gut tube spheroids generates HCOs that retain colonic identity after transplantation.

from Cell Stem Cell http://ift.tt/2txVigW
via Bhaskar Chanda UHN

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Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State

Bhaskar Chanda Stem Cell In this article, Deng and colleagues show that functional neurons and hepatocytes can be induced from fibroblasts via a chemically induced and highly expandable XEN-like state, bypassing the pluripotent stage. This featured lineage reprogramming strategy would be generalized for generating other functional desirable cell types.

from Cell Stem Cell http://ift.tt/2sYuUQr
via Bhaskar Chanda UHN

Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State

Bhaskar Chanda Stem Cell In this article, Deng and colleagues show that functional neurons and hepatocytes can be induced from fibroblasts via a chemically induced and highly expandable XEN-like state, bypassing the pluripotent stage. This featured lineage reprogramming strategy would be generalized for generating other functional desirable cell types.

from Cell Stem Cell http://ift.tt/2sYuUQr
via Bhaskar Chanda UHN

Dynamic Reorganization of Chromatin Accessibility Signatures during Dedifferentiation of Secretory Precursors into Lgr5+ Intestinal Stem Cells

Bhaskar Chanda Stem Cell Jadhav et al. identify an active enhancer signature that distinguishes Lgr5+ intestinal stem cells (ISCs) from Bmi1GFP+ and other secretory cells, including CD69+CD274+ goblet cell precursors. These specialized cells dedifferentiate into Lgr5+ ISCs in response to stem cell attrition, which is accompanied by dynamic rearrangements in open chromatin signatures.

from Cell Stem Cell http://ift.tt/2sYEJ0P
via Bhaskar Chanda UHN

Integrin α7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma

Bhaskar Chanda Stem Cell Haas et al. identify integrin α7 as a functional marker of glioblastoma stem cells by screening a monoclonal antibody library generated against primary glioblastoma (GBM) cells. Functional experiments in culture and in vivo show that the targeting of integrin α7 has potential as a therapeutic avenue for treating this aggressive disease.

from Cell Stem Cell http://ift.tt/2sHrRZp
via Bhaskar Chanda UHN

Integrin α7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma

Bhaskar Chanda Stem Cell Haas et al. identify integrin α7 as a functional marker of glioblastoma stem cells by screening a monoclonal antibody library generated against primary glioblastoma (GBM) cells. Functional experiments in culture and in vivo show that the targeting of integrin α7 has potential as a therapeutic avenue for treating this aggressive disease.

from Cell Stem Cell http://ift.tt/2sHrRZp
via Bhaskar Chanda UHN