Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia
Bhaskar Chanda Stem Cell Kotini et al. integrate patient cell reprogramming with mutational analysis to assemble a panel of iPSCs capturing distinct stages across the spectrum of myeloid malignancy. They use these cells to map transformation into transplantable leukemia and study both disease progression via CRISPR/Cas9 genome editing and stage-specific effects of therapeutic agents.
Cholinergic Signals from the CNS Regulate G-CSF-Mediated HSC Mobilization from Bone Marrow via a Glucocorticoid Signaling Relay
Bhaskar Chanda Stem Cell Pierce et al. show that the muscarinic receptor type-1 (Chrm1) expression in the brain regulates HSC mobilization from bone marrow via the hypothalamic-pituitary-adrenal axis, leading to glucocorticoid-mediated control of actin organization pathways.
Genome Editing in hPSCs Reveals Haploinsufficiency and a Genetic Interaction with in Human Pancreatic Development
Bhaskar Chanda Stem Cell Huangfu, Chen, and colleagues model human pancreatic disease by stepwise differentiation of genetically modified hPSCs to characterize the phenotypic effects of GATA6 haploinsufficiency not evident in mouse models and its genetic interaction with GATA4.