Single-Cell Analysis Reveals a Close Relationship between Differentiating Dopamine and Subthalamic Nucleus Neuronal Lineages
Bhaskar Chanda Stem Cell Kee et al. use single-cell RNA-seq to reconstruct Lmx1a+ differentiation in silico, revealing an unexpectedly close relationship between mesDA and STN neuronal lineages during differentiation. Application of markers that distinguish the two can help optimize mesDA differentiation of hESCs with a view to improving therapeutic translation.
Bhaskar Chanda Stem Cell Yao et al. perform single-cell RNA-seq during neural differentiation of hESCs. They identify many classes of neural progenitors and neurons that map to early human brain cells, computationally infer and experimentally confirm lineage relationships between them, and show that Wnt signaling influences the bifurcation between forebrain and mid/hindbrain lineages in vitro.
Predictive Markers Guide Differentiation to Improve Graft Outcome in Clinical Translation of hESC-Based Therapy for Parkinson’s Disease
Bhaskar Chanda Stem Cell Kirkeby et al. show that identification and application of a set of predictive markers can help refine differentiation protocols and improve transplant outcome in a preclinical model for hESC-based treatment of Parkinson’s disease.
Bhaskar Chanda Stem Cell Horsley and colleagues show that skin adipocyte stem cells are maintained and expanded through PDGFA, which activates a PI3K/AKT-signaling pathway to regulate gene expression. Loss of PDGFA expression during aging may well contribute to age-related loss of adipocyte stem cells and, thus, adipose tissue.