Bhaskar Chanda Stem Cell In vitro production of haploid gametes could provide a treatment for infertility, but recapitulating meiosis in culture is a significant roadblock. Zhou et al. report the generation of haploid male gametes from mouse ESCs that can produce viable and fertile offspring, demonstrating functional reproduction of meiosis in vitro.
Bhaskar Chanda Stem Cell Sharma, Ott, and colleagues show that expression of a key set of four transcription factors can reprogram hepatic myofibroblasts to induced hepatocyte-like cells in vivo and reduce liver fibrosis, suggesting that direct in vivo reprogramming may be an effective treatment approach for chronic liver disease.
Reprogrammed Stomach Tissue as a Renewable Source of Functional β Cells for Blood Glucose Regulation
Bhaskar Chanda Stem Cell Ariyachet et al. show that the antral stomach region of the gastrointestinal tract is particularly amenable to being reprogrammed to a β cell fate because of transcriptional similarity and that bioengineered stomach mini-organs containing reprogrammed cells can rescue hyperglycemia when transplanted into diabetic mice.
A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells
Bhaskar Chanda Stem Cell Young et al. demonstrate restoration of the DMD reading frame by CRISPR/Cas9-mediated deletion of up to 725 kb in hiPSCs as a therapeutic strategy for 60% of Duchenne muscular dystrophy patients. The resulting internally deleted protein is shown to be functional in vitro and in vivo.
Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors
Bhaskar Chanda Stem Cell Lalit et al. report that a combination of cardiac factors and signaling molecules reprogram adult mouse fibroblasts into expandable induced cardiac progenitor cells (iCPCs). iCPCs are multipotent and can differentiate into cardiomyocytes, smooth muscle, and endothelial cells. Moreover, iCPCs generate myocardium when injected into the embryonic and adult post-MI mouse heart.
Naive Human Pluripotent Cells Feature a Methylation Landscape Devoid of Blastocyst or Germline Memory
Bhaskar Chanda Stem Cell Pastor and colleagues show that reversion of primed hESCs in 5iLAF, or derivation of hESCs in 5iLAF, results in a population of naive cells characterized by loss of the marker SSEA4. However, these cells have a methylation pattern with little resemblance to blastocyst and near total loss of imprinting.