CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library

Bhaskar Chanda Stem Cell Li and colleagues show that the combined application of altered expression of two imprinted genes and CRISPR-Cas9-based genome editing allows the efficient and stable generation of gene-modified semi-cloned mice from androgenetic haploid embryonic stem cells. This approach has potential for mutagenesis and screening.

from Cell Stem Cell
via Bhaskar Chanda UHN


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