Archive | May 2015

Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches

Bhaskar Chanda Stem Cell Miki et al. develop synthetic miRNA switches for isolating cell populations that are otherwise difficult to purify. Several miRNA-responsive switches precisely and efficiently isolate human pluripotent stem cell (hPSC)-derived cardiomyocytes, and miRNA switches can be programmed for purification of various cell types including hPSC-derived endothelial cells, hepatocytes, and insulin-producing cells.

from Cell Stem Cell http://ift.tt/1PyOhEW
via Bhaskar Chanda UHN

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Combined Single-Cell Functional and Gene Expression Analysis Resolves Heterogeneity within Stem Cell Populations

Bhaskar Chanda Stem Cell Wilson et al. combine single-cell functional assays with flow cytometric index sorting and single-cell gene expression assays to reveal gene expression programs of HSCs with durable self-renewal potential in transplantation assays. They also demonstrate the broader applicability of this approach for linking key molecules with defined stem cell functions.

from Cell Stem Cell http://ift.tt/1R6SrB2
via Bhaskar Chanda UHN