Bhaskar Chanda Stem Cell Li et al. show that an ESC-enriched regulatory RNA, Apela, positively regulates p53-mediated DNA damage-induced apoptosis (DIA) of ESCs. Apela, hnRNPL, and p53 form a tri-element negative feedback loop that may help ESCs navigate the balance between maintaining inactive p53 under baseline conditions and eliciting DIA upon DNA damage.
Bhaskar Chanda Stem Cell Using Sox2-centered interactome analysis, Ding et al. identify Tex10 as an evolutionarily conserved key pluripotency factor that plays a critical role in ESC self-renewal and pluripotency, early embryo development, and somatic cell reprogramming via epigenetic regulation of super-enhancer activity.
Bhaskar Chanda Stem Cell Zhao et al. identify Filia as an ESC-specific regulator of genomic stability that is induced by genotoxic stress. Functional characterization highlights dynamic subcellular translocation and roles in governing multiple DNA damage response pathways, including stimulation of PARP1 enzymatic activity.
Erosion of X Chromosome Inactivation in Human Pluripotent Cells Initiates with Coating and Depends on a Specific Heterochromatin Landscape
Bhaskar Chanda Stem Cell Rougeulle and colleagues show that XCI erosion in human pluripotent cells is related to a pluripotency-specific epigenomic landscape on the inactive X chromosome, and that coating by the XACT lncRNA and gene reactivation precedes loss of XIST expression during this process.
β-Catenin Regulates Primitive Streak Induction through Collaborative Interactions with SMAD2/SMAD3 and OCT4
Bhaskar Chanda Stem Cell In this article, Funa, Schachter, and colleagues et al. provide mechanistic insights into how Wnt signaling controls early cell lineage decisions. β-catenin occupies regulatory regions in numerous PS and neural crest genes, and direct interactions between β-catenin and SMAD2/SMAD3 in proximity to OCT4 binding are required to specifically activate PS genes.
Opposing Roles for the lncRNA and Its Genomic Locus in Regulating Gene Activation during Embryonic Stem Cell Differentiation
Bhaskar Chanda Stem Cell Dissection of the precise contribution of RNA transcripts versus genomic DNA sequences is important for functional assessment of long noncoding RNAs. Here, Shen and colleagues demonstrate discrete and opposing roles for the lncRNA transcript Haunt and its genomic locus in regulating the HOXA gene cluster during embryonic stem cell differentiation.
Bhaskar Chanda Stem Cell Takebe et al. report a generalized method for organ bud formation from diverse tissues, including kidney, pancreas, intestine, heart, lung, and brain, using heterotypic cell mixtures including mesenchymal stem cells to guide cell condensation. After transplantation, renal and pancreatic buds are readily vascularized and exhibit tissue-specific organization and function.
Bhaskar Chanda Stem Cell Kida et al. show that upregulation of ERRα or ERRγ is required for a metabolic switch involving OXPHOS that is essential for iPSC generation and that early upregulation of ERRs marks cells that are destined to go on to be reprogrammed.